Recombinant human PCK1 protein

Phosphoenolpyruvate carboxykinase 1, also known as PCK1, is a main control point for the regulation of gluconeogenesis. PCK1 plays an important role in this process by stimulating hepatic glucose production. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Modulation of the signals governing PCK1 levels presents a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. Recombinant human PCK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxykinase 1, PEPCK-C, PEPCK1, PEPCKC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

71.7 kDa (646aa)

Additionnal Information

PCK1, Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxykinase 1, PEPCK-C, PEPCK1, PEPCKC, ATGP1372-10 µg, ATGP1372-20 µg, ATGP1372-50 µg, ATGP1372-100 µg, ATGP1372-250 µg, ATGP1372-500 µg, ATGP1372-1 mg, ATGP1372-10, ATGP1372-20, ATGP1372-50, ATGP1372-100, ATGP1372-250, ATGP1372-500, ATGP1372-1

References & Citations

Barthel A., et al. (2003) Am J Physiol Endocrinol Metab. 285:685-692.; ; Wang Y., et al. (1991) J Cell Physiol. 147:374-382.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

AAH23978.1

Uniprot Accession Number

P35558

Species

Human

AA Sequence

MPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM