Recombinant human RDH12 protein
Retinol dehydrogenase 12, also known as RDH12, belongs to the short-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed, mostly in eye, kidney, brain, skeletal muscle and stomach. It is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Recombinant human RDH12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Retinol dehydrogenase 12, LCA13, LCA3, SDR7C2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.5 kDa (303aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Maeda A., et al. (2006) J Biol Chem. 281:37697-37704.; ; Keller B., et al. (2007) J Steroid Biochem Mol Biol. 104:190-194.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 0.2M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_689656.2
Uniprot Accession Number
Q96NR8
Species
Human
AA Sequence
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