Recombinant human SRR protein
SRR, also known as serine racemase, is an enzyme which generates D-serine from L-serine. D-serine acts as a neuronal signaling molecule by activating NMDA receptors in the brain. Mammalian serine racemase is a pyridoxal 5'-phosphate dependent enzyme that catalyzes both the racemization of L-serine to D-serine and also the elimination of water from L-serine, generating pyruvate and ammonia. The enzyme is physiologically stimulated by divalent cations (e. g., magnesium) and is allosterically activated by the magnesium/ATP complex. Recombinant human SRR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Serine racemase, ILV1, ISO1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
39.1 kDa (364aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
De Miranda J., et al. (2000) Gene. 256:183-188; ; Smith M.A., et al. (2010) J. Biol. Chem. 285:12873-12881
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Neuroscience
NCBI Accession Number
NP_068766
Uniprot Accession Number
Q9GZT4
Species
Human
AA Sequence
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