Recombinant human TPM3 protein
Tropomyosin alpha-3 chain, also known as TPM3, is member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Recombinant human TPM3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tropomyosin alpha-3 chain, hscp30, NEM1, OK/SW-cl.5, TM-5, TM3, TM30 TM30 nm, TPMsk, TRK
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
31.6 kDa (272aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ohtsuki I., et al. (2002) J Biochem. 131:739-743.; ; Tiso N., et al. (1997) Biochem Biophys Res Commun. 230:347-350.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_705935.1
Uniprot Accession Number
P06753
Species
Human
AA Sequence
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