Recombinant human PHOSPHO2 protein
Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloacid dehalogenase (HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly. Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
30.3 kDa (265aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Roberts SJ., et al. (2005) Biochim Biophys Acta. 1752 (1) :73-82.; ; Stewart A J., et al. (2003) Protein Eng. 16 (12) :889-95.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001008489
Uniprot Accession Number
Q8TCD6
Species
Human
AA Sequence
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