Recombinant human PHOSPHO2 protein

Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloacid dehalogenase (HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly. Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30.3 kDa (265aa) confirmed by MALDI-TOF

Additionnal Information

PHOSPHO2, Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2, ATGP1367-10 µg, ATGP1367-20 µg, ATGP1367-50 µg, ATGP1367-100 µg, ATGP1367-250 µg, ATGP1367-500 µg, ATGP1367-1 mg, ATGP1367-10, ATGP1367-20, ATGP1367-50, ATGP1367-100, ATGP1367-250, ATGP1367-500, ATGP1367-1

References & Citations

Roberts SJ., et al. (2005) Biochim Biophys Acta. 1752 (1) :73-82.; ; Stewart A J., et al. (2003) Protein Eng. 16 (12) :889-95.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001008489

Uniprot Accession Number

Q8TCD6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKILLV FDFDNTIIDD NSDTWIVQCA PNKKLPIELR DSYRKGFWTE FMGRVFKYLG DKGVREHEMK RAVTSLPFTP GMVELFNFIR KNKDKFDCII ISDSNSVFID WVLEAASFHD IFDKVFTNPA AFNSNGHLTV ENYHTHSCNR CPKNLCKKVV LIEFVDKQLQ QGVNYTQIVY IGDGGNDVCP VTFLKNDDVA MPRKGYTLQK TLSRMSQNLE PMEYSVVVWS SGVDIISHLQ FLIKD