Recombinant human SNRPA1 protein

SNRPA1, also known as u2 small nuclear ribonucleoprotein A, is a component of the u2 snRNP that forms a complex with u2 snRNP B (u2B) . Together, u2 snRNP A and u2 snRNP B form a complex that binds to the u2 snRNA hairpin IV. The configuration of this u2 snRNP A/u2 snRNP B dimer and the subtle variations of a few key residues regulate the snRNP-RNA-binding specificity. u2 snRNP A is a 255 amino acid protein, and two isoforms exist as a result of alternative splicing events. Recombinant human SNRPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

U2 small nuclear ribonucleoprotein A, Lea1, small nuclear ribonucleoprotein polypeptide A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 80% by SDS-PAGE

Molecular Weight

30.5 kDa (275aa) confirmed by MALDI-TOF

Additionnal Information

SNRPA1, u2 small nuclear ribonucleoprotein A, Lea1, small nuclear ribonucleoprotein polypeptide A, ATGP1361-10 µg, ATGP1361-20 µg, ATGP1361-50 µg, ATGP1361-100 µg, ATGP1361-250 µg, ATGP1361-500 µg, ATGP1361-1 mg, ATGP1361-10, ATGP1361-20, ATGP1361-50, ATGP1361-100, ATGP1361-250, ATGP1361-500, ATGP1361-1

References & Citations

Crispino J.D. et al. (1994) Science 265: 1866-1869.; ; Price S.R. et al. (1998) Nature. 394: 645-650.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT, 0.1mM PMSF

Scientific Category

Immunology

NCBI Accession Number

NP_003081

Uniprot Accession Number

P09661

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK MFKGKRGAQL AKDIARRSKT FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD DGEEEMEEDT VTNGS