Recombinant human SNRPA1 protein
SNRPA1, also known as u2 small nuclear ribonucleoprotein A, is a component of the u2 snRNP that forms a complex with u2 snRNP B (u2B) . Together, u2 snRNP A and u2 snRNP B form a complex that binds to the u2 snRNA hairpin IV. The configuration of this u2 snRNP A/u2 snRNP B dimer and the subtle variations of a few key residues regulate the snRNP-RNA-binding specificity. u2 snRNP A is a 255 amino acid protein, and two isoforms exist as a result of alternative splicing events. Recombinant human SNRPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
U2 small nuclear ribonucleoprotein A, Lea1, small nuclear ribonucleoprotein polypeptide A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 80% by SDS-PAGE
Molecular Weight
30.5 kDa (275aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Crispino J.D. et al. (1994) Science 265: 1866-1869.; ; Price S.R. et al. (1998) Nature. 394: 645-650.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.2M NaCl,2mM DTT, 0.1mM PMSF
Scientific Category
Immunology
NCBI Accession Number
NP_003081
Uniprot Accession Number
P09661
Species
Human
AA Sequence
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