Recombinant human RTN4IP1 protein

RTN4IP1 is a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. Recombinant human RTN4IP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Reticulon-4-interacting protein 1, MGC12934, NIMP, Reticulon 4 interacting protein 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

41.4 kDa (379aa) confirmed by MALDI-TOF

Additionnal Information

RTN4IP1, Reticulon-4-interacting protein 1, MGC12934, NIMP, Reticulon 4 interacting protein 1, ATGP1360-10 µg, ATGP1360-20 µg, ATGP1360-50 µg, ATGP1360-100 µg, ATGP1360-250 µg, ATGP1360-500 µg, ATGP1360-1 mg, ATGP1360-10, ATGP1360-20, ATGP1360-50, ATGP1360-100, ATGP1360-250, ATGP1360-500, ATGP1360-1

References & Citations

Huber A.B. et al. (2000) Biol. Chem. 381: 407-419.; ; Hu W.H. et al. (2002) J. Neurochem. 81: 36-45.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_116119

Uniprot Accession Number

Q8WWV3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSTVMPAWV IDKYGKNEVL RFTQNMMMPI IHYPNEVIVK VHAASVNPID VNMRSGYGAT ALNMKRDPLH VKIKGEEFPL TLGRDVSGVV MECGLDVKYF KPGDEVWAAV PPWKQGTLSE FVVVSGNEVS HKPKSLTHTQ AASLPYVALT AWSAINKVGG LNDKNCTGKR VLILGASGGV GTFAIQVMKA WDAHVTAVCS QDASELVRKL GADDVIDYKS GSVEEQLKSL KPFDFILDNV GGSTETWAPD FLKKWSGATY VTLVTPFLLN MDRLGIADGM LQTGVTVGSK ALKHFWKGVH YRWAFFMASG PCLDDIAELV DAGKIRPVIE QTFPFSKVPE AFLKVERGHA RGKTVINVV