Recombinant human Spermine synthase/SMS protein

SMS (Spermine synthase) belongs to the spermidine/spermine synthase family. It is an enzyme that converts spermidine into spermine. This enzyme is required for normal viability, growth and fertility involved in polyamine metabolism. Defects in SMS are the cause of Snyder-Robinson syndrome (SRS), also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive. Recombinant human SMS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Spermine synthase, MRSR, SPMSY, SpS, SRS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

43.8 kDa (390aa) confirmed by MALDI-TOF

Additionnal Information

SMS, Spermine synthase, MRSR, SPMSY, SpS, SRS, ATGP1358-10 µg, ATGP1358-20 µg, ATGP1358-50 µg, ATGP1358-100 µg, ATGP1358-250 µg, ATGP1358-500 µg, ATGP1358-1 mg, ATGP1358-10, ATGP1358-20, ATGP1358-50, ATGP1358-100, ATGP1358-250, ATGP1358-500, ATGP1358-1

References & Citations

Cason A.L., et al. (2003) Eur. J. Hum. Genet. 11:937-944; ; Sowell J, et al. (2011) Clin Chim Acta. 18; ;412 (7-8) :655-60.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_004586.2

Uniprot Accession Number

P52788

Species

Human

AA Sequence

MAAARH STLDFMLGAK ADGETILKGL QSIFQEQGMA ESVHTWQDHG YLATYTNKNG SFANLRIYPH GLVLLDLQSY DGDAQGKEEI DSILNKVEER MKELSQDSTG RVKRLPPIVR GGAIDRYWPT ADGRLVEYDI DEVVYDEDSP YQNIKILHSK QFGNILILSG DVNLAESDLA YTRAIMGSGK EDYTGKDVLI LGGGDGGILC EIVKLKPKMV TMVEIDQMVI DGCKKYMRKT CGDVLDNLKG DCYQVLIEDC IPVLKRYAKE GREFDYVIND LTAVPISTSP EEDSTWEFLR LILDLSMKVL KQDGKYFTQG NCVNLTEALS LYEEQLGRLY CPVEFSKEIV CVPSYLELWV FYTVWKKAKP