Recombinant human SARS protein

SARS (Seryl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. This enzyme catalyzes the attachment of serine to tRNA (Ser) . It is probably able to aminoacylate tRNA (Sec) with serine, to form the misacylated tRNA L-seryl-tRNA (Sec), which will be further converted into selenocysteinyl-tRNA (Sec) . Recombinant human SARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Seryl-tRNA synthetase, SERRS, SERS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

61.2 kDa (537aa)

Additionnal Information

SARS, Seryl-tRNA synthetase, SERRS, SERS, Sars-CoV-2 S1, Sars-CoV-2 S1, covid, corona, ATGP1357-10 µg, ATGP1357-20 µg, ATGP1357-50 µg, ATGP1357-100 µg, ATGP1357-250 µg, ATGP1357-500 µg, ATGP1357-1 mg, ATGP1357-10, ATGP1357-20, ATGP1357-50, ATGP1357-100, ATGP1357-250, ATGP1357-500, ATGP1357-1

References & Citations

Hartlein M, et al. (1995) J. Mol. Evol. 40 (5) : 519-30.; ; Vincent C., et al. (1997) Eur. J. Biochem. 250:77-84

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_006504

Uniprot Accession Number

P49591

Species

Human

AA Sequence

MVLDLDL FRVDKGGDPA LIRETQEKRF KDPGLVDQLV KADSEWRRCR FRADNLNKLK NLCSKTIGEK MKKKEPVGDD ESVPENVLSF DDLTADALAN LKVSQIKKVR LLIDEAILKC DAERIKLEAE RFENLREIGN LLHPSVPISN DEDVDNKVER IWGDCTVRKK YSHVDLVVMV DGFEGEKGAV VAGSRGYFLK GVLVFLEQAL IQYALRTLGS RGYIPIYTPF FMRKEVMQEV AQLSQFDEEL YKVIGKGSEK SDDNSYDEKY LIATSEQPIA ALHRDEWLRP EDLPIKYAGL STCFRQEVGS HGRDTRGIFR VHQFEKIEQF VYSSPHDNKS WEMFEEMITT AEEFYQSLGI PYHIVNIVSG SLNHAASKKL DLEAWFPGSG AFRELVSCSN CTDYQARRLR IRYGQTKKMM DKVEFVHMLN ATMCATTRTI CAILENYQTE KGITVPEKLK EFMPPGLQEL IPFVKPAPIE QEPSKKQKKQ HEGSKKKAAA RDVTLENRLQ NMEVTDA