Recombinant human RPIA protein
RPIA (Ribose-5-phosphate isomerase) belongs to the ribose 5-phosphate isomerase family. RPIA is an enzyme that catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5-phosphate (Ru5P) . RPI exists as two distinct proteins forms, termed RPIA and RPIB. RPIA plays an essential role in the metabolism of plants and animals, as it is involved in the Calvin cycle which takes place in plants, and the pentose phosphate pathway which takes place in plants as well as animals. Recombinant human RPIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ribose 5-phosphate isomerase, Phosphoriboisomerase, RPI
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.4 kDa (331aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhang, R., et al. (2003) Structure 11 (1) : 31.; ; Huck J.H.J., et al. (2004) Am. J. Hum. Genet. 74:745-751
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl, 2mM EDTA, 0,2mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
NP_653164.2
Uniprot Accession Number
P49247
Species
Human
AA Sequence
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