Recombinant human APPBP1/NAE1 protein
NAE1 (NEDD8-activating enzyme E1 regulatory subunit) belongs to the ubiquitin-activating E1 family. This protein binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. NAE1 participates in a novel ubiquitinylation-related pathway involving the ubiquitin-like molecule NEDD8. Also, this protein is required for cell cycle progression through the S/M checkpoint. Recombinant human NAE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
NEDD8 activating enzyme E1 subunit 1, A-116A10.1, APPBP1, HPP1, ula-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
62.7 kDa (557aa)
Additionnal Information
References & Citations
Chen Y, et al. (2000) J Biol Chem. 275 (12) :8929-35.; ; Bohnsack R.N., et al. (2003) J. Biol. Chem. 278:26823-26830
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003896
Uniprot Accession Number
Q13564
Species
Human
AA Sequence
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