Recombinant human MVD protein
MVD, also known as diphosphomevalonate decarboxylase, catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Recombinant human MVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Diphosphomevalonate decarboxylase, MPD
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
45.6 kDa (420aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Toth M.J., et al. (1996) J. Biol. Chem. 271:7895-7898; ; Molina H., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:2199-2204
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_002452
Uniprot Accession Number
P53602
Species
Human
AA Sequence
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