Recombinant human MVD protein

MVD, also known as diphosphomevalonate decarboxylase, catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Recombinant human MVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Diphosphomevalonate decarboxylase, MPD

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

45.6 kDa (420aa) confirmed by MALDI-TOF

Additionnal Information

MVD, diphosphomevalonate decarboxylase, MPD, ATGP1354-10 µg, ATGP1354-20 µg, ATGP1354-50 µg, ATGP1354-100 µg, ATGP1354-250 µg, ATGP1354-500 µg, ATGP1354-1 mg, ATGP1354-10, ATGP1354-20, ATGP1354-50, ATGP1354-100, ATGP1354-250, ATGP1354-500, ATGP1354-1

References & Citations

Toth M.J., et al. (1996) J. Biol. Chem. 271:7895-7898; ; Molina H., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:2199-2204

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_002452

Uniprot Accession Number

P53602

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASEKPLAAV TCTAPVNIAV IKYWGKRDEE LVLPINSSLS VTLHQDQLKT TTTAVISKDF TEDRIWLNGR EEDVGQPRLQ ACLREIRCLA RKRRNSRDGD PLPSSLSCKV HVASVNNFPT AAGLASSAAG YACLAYTLAR VYGVESDLSE VARRGSGSAC RSLYGGFVEW QMGEQADGKD SIARQVAPES HWPELRVLIL VVSAEKKLTG STVGMRASVE TSPLLRFRAE SVVPARMAEM ARCIRERDFP SFAQLTMKDS NQFHATCLDT FPPISYLNAI SWRIIHLVHR FNAHHGDTKV AYTFDAGPNA VIFTLDDTVA EFVAAVWHGF PPGSNGDTFL KGLQVRPAPL SAELQAALAM EPTPGGVKYI IVTQVGPGPQ ILDDPCAHLL GPDGLPKPAA