Recombinant human PSMF1 protein

PSMF1 is a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Proteasome inhibitor subunit 1 isoform 1, PI31

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

31.9 kDa (291aa) confirmed by MALDI-TOF

Additionnal Information

PSMF1, Proteasome inhibitor subunit 1 isoform 1, PI31, ATGP1352-10 µg, ATGP1352-20 µg, ATGP1352-50 µg, ATGP1352-100 µg, ATGP1352-250 µg, ATGP1352-500 µg, ATGP1352-1 mg, ATGP1352-10, ATGP1352-20, ATGP1352-50, ATGP1352-100, ATGP1352-250, ATGP1352-500, ATGP1352-1

References & Citations

Tanahashi N. et al. (1999) Mol Biol Rep 26: 3-9.; ; Seeger M. et al. (1997) J. Biol. Chem. 272: 8145-8148.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT,0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_006805

Uniprot Accession Number

Q92530

Species

Human

AA Sequence

MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYCGLGV GDQPGPNDKK SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG TSPPGPNPDH LPPPGYDDMY L