Recombinant human PSMB6 protein
PSMB6 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMB6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome subunit beta type-6 precursor, DELTA, LMPY, MGC5169
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
24.2 kDa (226aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Apcher GS., et al. (2003) FEBS Lett. 553 (1-2) :200-4.; ; Stelzl u., et al. (2002) Cell. 122 (6) :957-68.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002789
Uniprot Accession Number
P28072
Species
Human
AA Sequence
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