Recombinant human PSMA4 protein
PSMA4, also known as proteasome subunit alpha type 4, is a member of the peptidase T1A family, that is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Proteasome subunit alpha type-4 isoform 1, HC9, HsT17706, MGC111191, MGC12467, MGC24813, PSC9
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
32.0 kDa (285aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kristensen P. et al. (1995) Biochem. Biophys. Res.Commun. 205: 1785-1789.; ; Morimoto Y. et al. (1995) J. Biochem. 117: 471-474
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001096137
Uniprot Accession Number
P25789
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CDS1 Human shRNA Lentiviral Particle (Locus ID 1040)
Rat Adrenergic Receptor Alpha 1A (ADRa1A) ELISA Kit