Recombinant human PSMA4 protein

PSMA4, also known as proteasome subunit alpha type 4, is a member of the peptidase T1A family, that is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Proteasome subunit alpha type-4 isoform 1, HC9, HsT17706, MGC111191, MGC12467, MGC24813, PSC9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

32.0 kDa (285aa) confirmed by MALDI-TOF

Additionnal Information

PSMA4, Proteasome subunit alpha type-4 isoform 1, HC9, HsT17706, MGC111191, MGC12467, MGC24813, PSC9, ATGP1350-10 µg, ATGP1350-20 µg, ATGP1350-50 µg, ATGP1350-100 µg, ATGP1350-250 µg, ATGP1350-500 µg, ATGP1350-1 mg, ATGP1350-10, ATGP1350-20, ATGP1350-50, ATGP1350-100, ATGP1350-250, ATGP1350-500, ATGP1350-1

References & Citations

Kristensen P. et al. (1995) Biochem. Biophys. Res.Commun. 205: 1785-1789.; ; Morimoto Y. et al. (1995) J. Biochem. 117: 471-474

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001096137

Uniprot Accession Number

P25789

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSRRYD SRTTIFSPEG RLYQVEYAME AIGHAGTCLG ILANDGVLLA AERRNIHKLL DEVFFSEKIY KLNEDMACSV AGITSDANVL TNELRLIAQR YLLQYQEPIP CEQLVTALCD IKQAYTQFGG KRPFGVSLLY IGWDKHYGFQ LYQSDPSGNY GGWKATCIGN NSAAAVSMLK QDYKEGEMTL KSALALAIKV LNKTMDVSKL SAEKVEIATL TRENGKTVIR VLKQKEVEQL IKKHEEEEAK AEREKKEKEQ KEKDK