Recombinant human PGK2 protein

PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46.9 kDa (437aa) confirmed by MALDI-TOF

Additionnal Information

PGK2, Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS, ATGP1349-10 µg, ATGP1349-20 µg, ATGP1349-50 µg, ATGP1349-100 µg, ATGP1349-250 µg, ATGP1349-500 µg, ATGP1349-1 mg, ATGP1349-10, ATGP1349-20, ATGP1349-50, ATGP1349-100, ATGP1349-250, ATGP1349-500, ATGP1349-1

References & Citations

Svaasand EK. et al. (2007) Muscle Nerve. 36:679-84.; ; Valentin C. et al. (1998) Hum Mutat. 12 (4) :280-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_620061

Uniprot Accession Number

P07205

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM