Recombinant human PGK2 protein
PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
46.9 kDa (437aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Svaasand EK. et al. (2007) Muscle Nerve. 36:679-84.; ; Valentin C. et al. (1998) Hum Mutat. 12 (4) :280-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_620061
Uniprot Accession Number
P07205
Species
Human
AA Sequence
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