Recombinant human PCYT2 protein

PCYT2 is an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. Recombinant human PCYT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Ethanolamine-phosphate cytidylyltransferase isoform 2, CTP:phosphoethanolamine cytidylyltransferase, ET, Ethanolamine-phosphate cytidylyltransferase, Phosphorylethanolamine transferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.9 kDa (409aa) confirmed by MALDI-TOF

Additionnal Information

PCYT2, Ethanolamine-phosphate cytidylyltransferase isoform 2, CTP:phosphoethanolamine cytidylyltransferase, ET, Ethanolamine-phosphate cytidylyltransferase, Phosphorylethanolamine transferase, ATGP1348-10 µg, ATGP1348-20 µg, ATGP1348-50 µg, ATGP1348-100 µg, ATGP1348-250 µg, ATGP1348-500 µg, ATGP1348-1 mg, ATGP1348-10, ATGP1348-20, ATGP1348-50, ATGP1348-100, ATGP1348-250, ATGP1348-500, ATGP1348-1

References & Citations

Nakashima A. et al. (1997) J. Biol. Chem. 272: 9567-9572.; ; Bakovic M. et al. (2007) Biochem. Cell Biol. 85:283-300.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_002852

Uniprot Accession Number

Q99447

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD YLIVGVHTDE EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE TLDKYNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV GRMLLVTKAH HSSQEMSSEY REYADSFGKC PGGRNPWTGV SQFLQTSQKI IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA ERPYIIAGLH FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII TNRLEYEARN QKKEAKELAF LEAARQQAAQ PLGERDGDF