Recombinant human NEIL1 protein

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Recombinant human NEIL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Endonuclease 8-like 1, FLJ22402, FPG1, hFPG1, NEI1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.8 kDa (410aa) confirmed by MALDI-TOF

Additionnal Information

NEIL1, Endonuclease 8-like 1, FLJ22402, FPG1, hFPG1, NEI1, ATGP1347-10 µg, ATGP1347-20 µg, ATGP1347-50 µg, ATGP1347-100 µg, ATGP1347-250 µg, ATGP1347-500 µg, ATGP1347-1 mg, ATGP1347-10, ATGP1347-20, ATGP1347-50, ATGP1347-100, ATGP1347-250, ATGP1347-500, ATGP1347-1

References & Citations

Hazra T.K. et al. (2002) J. Biol.Chem. 277: 30417-30420.; ; Dou H. et al. (2003) J. Biol.Chem. 278: 49679-49684.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_078884.2

Uniprot Accession Number

Q96FI4

Species

Human

AA Sequence

MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS