Recombinant human NEIL1 protein
NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Recombinant human NEIL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Endonuclease 8-like 1, FLJ22402, FPG1, hFPG1, NEI1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.8 kDa (410aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hazra T.K. et al. (2002) J. Biol.Chem. 277: 30417-30420.; ; Dou H. et al. (2003) J. Biol.Chem. 278: 49679-49684.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_078884.2
Uniprot Accession Number
Q96FI4
Species
Human
AA Sequence
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