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Recombinant human LCMT1 protein

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A. Recombinant human LCMT7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Leucine carboxyl methyltransferase 1, CGI-68, LCMT, PPMT1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

40.9 kDa (358aa) confirmed by MALDI-TOF

Additionnal Information

LCMT1, Leucine carboxyl methyltransferase 1, CGI-68, LCMT, PPMT1, ATGP1346-10 µg, ATGP1346-20 µg, ATGP1346-50 µg, ATGP1346-100 µg, ATGP1346-250 µg, ATGP1346-500 µg, ATGP1346-1 mg, ATGP1346-10, ATGP1346-20, ATGP1346-50, ATGP1346-100, ATGP1346-250, ATGP1346-500, ATGP1346-1

References & Citations

Lee JA., et al. (2007) J Biol Chem. 282 (42) :30974-84.; ; De Baere I., et al. (1999) Biochemistry. 38 (50) :16539-47.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Scientific Category

Cancer

NCBI Accession Number

NP_057393

Uniprot Accession Number

Q9UIC8

Species

Human

AA Sequence

MATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY

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