Recombinant human LCMT1 protein
LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A. Recombinant human LCMT7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Leucine carboxyl methyltransferase 1, CGI-68, LCMT, PPMT1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
40.9 kDa (358aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lee JA., et al. (2007) J Biol Chem. 282 (42) :30974-84.; ; De Baere I., et al. (1999) Biochemistry. 38 (50) :16539-47.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
Scientific Category
Cancer
NCBI Accession Number
NP_057393
Uniprot Accession Number
Q9UIC8
Species
Human
AA Sequence
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