Recombinant human HMGCL protein
HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA. Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Hydroxymethylglutaryl-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HL, HMG-CoA lyase, Hydroxymethylglutaryl-CoA lyase mitochondrial
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
34.2 kDa (323aa)
Additionnal Information
References & Citations
Cardoso M.L. et al. (2004) Mol. Genet. Metab. 82: 334-338.; ; Wang S.P et al. (1996) Genomics 33: 99-104.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Scientific Category
Cancer
NCBI Accession Number
NP_000182
Uniprot Accession Number
P35914
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Monoclonal Anti-Adenovirus (many isotypes) IgG-FITC conjugate
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
