Recombinant human DHRS9 protein

DHRS9, also known as dehydrogenase/reductase SDR family member 9, functions as a homotetramer that converts both 3-alpha-tetrahydroprogesterone (allopregnanolone) and 3-alpha-androstanediol to dihydroxyprogesterone and is thought to play a role in retinoic acid biosynthesis. Recombinant human DHRS9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, 3-alpha-HSD, 3ALPHA-HSD, 3alpha-HSD, Dehydrogenase/reductase SDR family member 9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.9 kDa (327aa) confirmed by MALDI-TOF

Additionnal Information

DHRS9, Dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, 3-alpha-HSD, 3ALPHA-HSD, 3alpha-HSD, Dehydrogenase/reductase SDR family member 9, ATGP1343-10 µg, ATGP1343-20 µg, ATGP1343-50 µg, ATGP1343-100 µg, ATGP1343-250 µg, ATGP1343-500 µg, ATGP1343-1 mg, ATGP1343-10, ATGP1343-20, ATGP1343-50, ATGP1343-100, ATGP1343-250, ATGP1343-500, ATGP1343-1

References & Citations

Chetyrkin S.V. et al. (2001) J. Biol.Chem. 276: 22278-22286.; ; Jones R.J. et al. (2007) . J. Biol.Chem. 282: 8317-8324.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

NP_005762

Uniprot Accession Number

Q9BPW9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMRKGKL KIEDITDKYI FITGCDSGFG NLAARTFDKK GFHVIAACLT ESGSTALKAE TSERLRTVLL DVTDPENVKR TAQWVKNQVG EKGLWGLINN AGVPGVLAPT DWLTLEDYRE PIEVNLFGLI SVTLNMLPLV KKAQGRVINV SSVGGRLAIV GGGYTPSKYA VEGFNDSLRR DMKAFGVHVS CIEPGLFKTN LADPVKVIEK KLAIWEQLSP DIKQQYGEGY IEKSLDKLKG NKSYVNMDLS PVVECMDHAL TSLFPKTHYA AGKDAKIFWI PLSHMPAALQ DFLLLKQKAE LANPKAV