Recombinant human DHRS9 protein
DHRS9, also known as dehydrogenase/reductase SDR family member 9, functions as a homotetramer that converts both 3-alpha-tetrahydroprogesterone (allopregnanolone) and 3-alpha-androstanediol to dihydroxyprogesterone and is thought to play a role in retinoic acid biosynthesis. Recombinant human DHRS9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.9 kDa (327aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chetyrkin S.V. et al. (2001) J. Biol.Chem. 276: 22278-22286.; ; Jones R.J. et al. (2007) . J. Biol.Chem. 282: 8317-8324.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
NP_005762
Uniprot Accession Number
Q9BPW9
Species
Human
AA Sequence
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