Recombinant human CCND2 protein

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of this protein was observed in ovarian and testicular tumors. Recombinant human CCND2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cyclin D2, KIAK0002, MGC102758, G1/S-specific cyclin-D2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.6 kDa (313aa)

Additionnal Information

CCND2, Cyclin D2, KIAK0002, MGC102758, G1/S-specific cyclin-D2, ATGP1342-10 µg, ATGP1342-20 µg, ATGP1342-50 µg, ATGP1342-100 µg, ATGP1342-250 µg, ATGP1342-500 µg, ATGP1342-1 mg, ATGP1342-10, ATGP1342-20, ATGP1342-50, ATGP1342-100, ATGP1342-250, ATGP1342-500, ATGP1342-1

References & Citations

Inaba T., et al. (1992) Genomics. 13 (3) :565-74.; ; Wang Z., et al. (2008) Cell Struct Funct. 33 (2) :171-83.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 1M urea.

Scientific Category

Cancer

NCBI Accession Number

NP_001750

Uniprot Accession Number

P30279

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMELLCH EVDPVRRAVR DRNLLRDDRV LQNLLTIEER YLPQCSYFKC VQKDIQPYMR RMVATWMLEV CEEQKCEEEV FPLAMNYLDR FLAGVPTPKS HLQLLGAVCM FLASKLKETS PLTAEKLCIY TDNSIKPQEL LEWELVVLGK LKWNLAAVTP HDFIEHILRK LPQQREKLSL IRKHAQTFIA LCATDFKFAM YPPSMIATGS VGAAICGLQQ DEEVSSLTCD ALTELLAKIT NTDVDCLKAC QEQIEAVLLN SLQQYRQDQR DGSKSEDELD QASTPTDVRD IDL