Recombinant human BDH1 protein

BDH1 is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R) -3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Recombinant human BDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.3 kDa (318aa) confirmed by MALDI-TOF

Additionnal Information

BDH1, D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1, ATGP1341-10 µg, ATGP1341-20 µg, ATGP1341-50 µg, ATGP1341-100 µg, ATGP1341-250 µg, ATGP1341-500 µg, ATGP1341-1 mg, ATGP1341-10, ATGP1341-20, ATGP1341-50, ATGP1341-100, ATGP1341-250, ATGP1341-500, ATGP1341-1

References & Citations

Marks A.R. et al. (1992) J Biol Chem. 267:15459-15463.; ; Adami P. et al. (1993) Biochem. J. 292: 863-872.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.2M NaCl,2mM DTT, 0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

NP_976059

Uniprot Accession Number

Q02338

Species

Human

AA Sequence

ASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVC SSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPAR SPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDT SPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR