Recombinant human BDH1 protein
BDH1 is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R) -3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Recombinant human BDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
35.3 kDa (318aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Marks A.R. et al. (1992) J Biol Chem. 267:15459-15463.; ; Adami P. et al. (1993) Biochem. J. 292: 863-872.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.2M NaCl,2mM DTT, 0.1mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
NP_976059
Uniprot Accession Number
Q02338
Species
Human
AA Sequence
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