Recombinant human VAT1 protein

VAT1 (Synaptic vesicle membrane protein VAT-1 homolog) belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. This protein increased expression in glioblastomas and on wounding, in basal keratinocytes. This expression is calcium ion-dependent. Recombinant human VAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Synaptic vesicle membrane protein VAT-1 homolog, VATI, FLJ20230, vesicle amine transport protein 1 homolog

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.1 kDa (413aa) confirmed by MALDI-TOF

Additionnal Information

VAT1, Synaptic vesicle membrane protein VAT-1 homolog, VATI, FLJ20230, vesicle amine transport protein 1 homolog, ATGP1339-10 µg, ATGP1339-20 µg, ATGP1339-50 µg, ATGP1339-100 µg, ATGP1339-250 µg, ATGP1339-500 µg, ATGP1339-1 mg, ATGP1339-10, ATGP1339-20, ATGP1339-50, ATGP1339-100, ATGP1339-250, ATGP1339-500, ATGP1339-1

References & Citations

Koch J., et al. (2003) Arch. Dermatol. Res. 295:203-210; ; Mertsch S., et al. (2009) Neuropathol. Appl. Neurobiol. 35:342-352

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_006364

Uniprot Accession Number

Q99536

Species

Human

AA Sequence

MSDEREVAEA ATGEDASSPP PKTEAASDPQ HPAASEGAAA AAASPPLLRC LVLTGFGGYD KVKLQSRPAA PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA VGEGVSDRKA GDRVMVLNRS GMWQEEVTVP SVQTFLIPEA MTFEEAAALL VNYITAYMVL FDFGNLQPGH SVLVHMAAGG VGMAAVQLCR TVENVTVFGT ASASKHEALK ENGVTHPIDY HTTDYVDEIK KISPKGVDIV MDPLGGSDTA KGYNLLKPMG KVVTYGMANL LTGPKRNLMA LARTWWNQFS VTALQLLQAN RAVCGFHLGY LDGEVELVSG VVARLLALYN QGHIKPHIDS VWPFEKVADA MKQMQEKKNV GKVLLVPGPE KEN