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Recombinant human ARL9 protein

ARL9 (ADP-ribosylation factor-like protein 9) belongs to two novel subfamilies coding for small GTPases that we found to be highly conserved among eukaryotes. Recombinant human ARL9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

ARL9, ADP ribosylation factor like protein 9, ADP ribosylation factor like GTPase 9, ADP ribosylation factor like 9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.9 kDa (143aa) confirmed by MALDI-TOF

Additionnal Information

ARL9, ADP-ribosylation factor-like 9, ADP ribosylation factor like protein 9, ADP ribosylation factor like GTPase 9, ADP ribosylation factor like 9, ATGP1337-10 µg, ATGP1337-20 µg, ATGP1337-50 µg, ATGP1337-100 µg, ATGP1337-250 µg, ATGP1337-500 µg, ATGP1337-1 mg, ATGP1337-010, ATGP1337-020, ATGP1337-050, ATGP1337-100, ATGP1337-250, ATGP1337-500, ATGP1337-01M

References & Citations

Louro R, et al. (2004) Biochem Biophys Res Commun. 316 (3) :618-27.; ; Pasqualato S., et al (2002) EMBO Rep. 3 (11) :1035-41.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 200 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_996802

Uniprot Accession Number

Q6T311

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEFLEIGGSK PFRSYWEMYL SKGLLLIFVV DSADHSRLPE AKKYLHQLIA ANPVLPLVVF ANKQDLEAAY HITDIHEALA LSEVGNDRKM FLFGTYLTKN GSEIPSTMQD AKDLIAQLAA DVQ
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