Recombinant human PSMA5 protein

PSMA5 (Proteasome subunit alpha type-5) belongs to the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 has been shown to interact with PLK1. This peptidase has a very broad specificity for cleavage of peptide bonds. Recombinant human PSMA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome subunit alpha type-5, PSC5, ZETA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.5 kDa (277aa) confirmed by MALDI-TOF

Additionnal Information

PSMA5, Proteasome subunit alpha type-5, PSC5, ZETA, ATGP1336-10 µg, ATGP1336-20 µg, ATGP1336-50 µg, ATGP1336-100 µg, ATGP1336-250 µg, ATGP1336-500 µg, ATGP1336-1 mg, ATGP1336-10, ATGP1336-20, ATGP1336-50, ATGP1336-100, ATGP1336-250, ATGP1336-500, ATGP1336-1

References & Citations

Feng, Y., et al. (2001) Cell Growth Differ. (united States) 12 (1) : 29-37.; ; Hirano Y., et al. (2005) Nature 437:1381-1385

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002781

Uniprot Accession Number

P28066

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMFLT RSEYDRGVNT FSPEGRLFQV EYAIEAIKLG STAIGIQTSE GVCLAVEKRI TSPLMEPSSI EKIVEIDAHI GCAMSGLIAD AKTLIDKARV ETQNHWFTYN ETMTVESVTQ AVSNLALQFG EEDADPGAMS RPFGVALLFG GVDEKGPQLF HMDPSGTFVQ CDARAIGSAS EGAQSSLQEV YHKSMTLKEA IKSSLIILKQ VMEEKLNATN IELATVQPGQ NFHMFTKEEL EEVIKDI