Recombinant human PSMA5 protein
PSMA5 (Proteasome subunit alpha type-5) belongs to the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 has been shown to interact with PLK1. This peptidase has a very broad specificity for cleavage of peptide bonds. Recombinant human PSMA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome subunit alpha type-5, PSC5, ZETA
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30.5 kDa (277aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Feng, Y., et al. (2001) Cell Growth Differ. (united States) 12 (1) : 29-37.; ; Hirano Y., et al. (2005) Nature 437:1381-1385
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002781
Uniprot Accession Number
P28066
Species
Human
AA Sequence
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