Recombinant human RRAS3/MRAS protein

MRAS (Ras-related protein M-Ras) belongs to the RAS superfamily. These families are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. MRAS serve as an important signal transducer for novel upstream stimuli in controlling cell proliferation. Recombinant human MRAS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein M-Ras, M-RAs, R-RAS3, RRAS3, Muscle RAS oncogene homolog, Ras-related protein R-Ras3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.7 kDa (225aa) confirmed by MALDI-TOF

Additionnal Information

M-Ras, R-Ras3, Ras-related protein M-Ras, M-RAs, R-RAS3, RRAS3, ATGP1335-10 µg, ATGP1335-20 µg, ATGP1335-50 µg, ATGP1335-100 µg, ATGP1335-250 µg, ATGP1335-500 µg, ATGP1335-1 mg, ATGP1335-10, ATGP1335-20, ATGP1335-50, ATGP1335-100, ATGP1335-250, ATGP1335-500, ATGP1335-1

References & Citations

Rodriguez-Viciana P., et al (2006) Mol. Cell 22:217-230; ; Quilliam LA, et al. (1999) J Biol Chem 274 (34) : 23850-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 40% glycerol, 200 mM NaCl, 2mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036351.3

Uniprot Accession Number

O14807

Species

Human

AA Sequence

MATSAVPSDN LPTYKLVVVG DGGVGKSALT IQFFQKIFVP DYDPTIEDSY LKHTEIDNQW AILDVLDTAG QEEFSAMREQ YMRTGDGFLI VYSVTDKASF EHVDRFHQLI LRVKDRESFP MILVANKVDL MHLRKITREQ GKEMATKHNI PYIETSAKDP PLNVDKAFHD LVRVIRQQIP EKSQKKKKKT KWRGDRATGT HKLQC