Recombinant human eIF-4A3/EIF4A3 protein
EIF4A3 (Eukaryotic initiation factor 4A-III) belongs to the DEAD box helicase family and eIF4A subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. EIF4A3 is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. Recombinant human EIF4A3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
49.4 kDa (435aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chan C.C., et al. (2004) RNA 10:200-209 ; ; Li Q, et al. (1999) . Mol. Cell. Biol. 19 (11) : 7336-46.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 200 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_055555
Uniprot Accession Number
P38919
Species
Human
AA Sequence
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