Recombinant human CTH protein

CTH (Cystathionine gamma-lyase or cystathionase) belongs to the trans-sulfuration enzymes family. This protein is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. CTH catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Recombinant human CTH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cystathionine gamma-lyase, Cystathionine gamma-lyase, Gamma-cystathionase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

46.7 kDa (425aa) confirmed by MALDI-TOF

Additionnal Information

CTH, Cystathionine gamma-lyase, Cystathionine gamma-lyase, Gamma-cystathionase, ATGP1333-10 µg, ATGP1333-20 µg, ATGP1333-50 µg, ATGP1333-100 µg, ATGP1333-250 µg, ATGP1333-500 µg, ATGP1333-1 mg, ATGP1333-10, ATGP1333-20, ATGP1333-50, ATGP1333-100, ATGP1333-250, ATGP1333-500, ATGP1333-1

References & Citations

Chiku T., et al. (2009) J. Biol. Chem. 284:11601-11612; ; Sun Q., et al. (2009) J. Biol. Chem. 284:3076-3085

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_001893.2

Uniprot Accession Number

P32929

Species

Human

AA Sequence

MQEKDASSQG FLPHFQHFAT QAIHVGQDPE QWTSRAVVPP ISLSTTFKQG APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYCLAFASG LAATVTITHL LKAGDQIICM DDVYGGTNRY FRQVASEFGL KISFVDCSKI KLLEAAITPE TKLVWIETPT NPTQKVIDIE GCAHIVHKHG DIILVVDNTF MSPYFQRPLA LGADISMYSA TKYMNGHSDV VMGLVSVNCE SLHNRLRFLQ NSLGAVPSPI DCYLCNRGLK TLHVRMEKHF KNGMAVAQFL ESNPWVEKVI YPGLPSHPQH ELVKRQCTGC TGMVTFYIKG TLQHAEIFLK NLKLFTLAES LGGFESLAEL PAIMTHASVL KNDRDVLGIS DTLIRLSVGL EDEEDLLEDL DQALKAAHPP SGSHS