Recombinant human DECR2 protein

DECR2, also known as peroxisomal 2, 4-dienoyl-CoA reductase, is an auxiliary enzyme of beta-oxidation. DECR2 participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. This protein catalyzes the NADP-dependent reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA. Recombinant human DECR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Peroxisomal 24-dienoyl-CoA reductase, Peroxisomal 2,4-dienoyl-CoA reductase, PDCR, SDR17C1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.2 kDa (315aa) confirmed by MALDI-TOF

Additionnal Information

DECR2, Peroxisomal 24-dienoyl-CoA reductase, Peroxisomal 2,4-dienoyl-CoA reductase, PDCR, SDR17C1, ATGP1330-10 µg, ATGP1330-20 µg, ATGP1330-50 µg, ATGP1330-100 µg, ATGP1330-250 µg, ATGP1330-500 µg, ATGP1330-1 mg, ATGP1330-10, ATGP1330-20, ATGP1330-50, ATGP1330-100, ATGP1330-250, ATGP1330-500, ATGP1330-1

References & Citations

De Nys K., et al. (2001) Biochim. Biophys. Acta. 1533:66-72; ; Daniels R.J., et al. (2001) Hum. Mol. Genet. 10:339-352

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_065715

Uniprot Accession Number

Q9NUI1

Species

Human

AA Sequence

MAQPPPD VEGDDCLPAY RHLFCPDLLR DKVAFITGGG SGIGFRIAEI FMRHGCHTVI ASRSLPRVLT AARKLAGATG RRCLPLSMDV RAPPAVMAAV DQALKEFGRI DILINCAAGN FLCPAGALSF NAFKTVMDID TSGTFNVSRV LYEKFFRDHG GVIVNITATL GNRGQALQVH AGSAKAAVDA MTRHLAVEWG PQNIRVNSLA PGPISGTEGL RRLGGPQASL STKVTASPLQ RLGNKTEIAH SVLYLASPLA SYVTGAVLVA DGGAWLTFPN GVKGLPDFAS FSAKL