Recombinant human DECR2 protein
DECR2, also known as peroxisomal 2, 4-dienoyl-CoA reductase, is an auxiliary enzyme of beta-oxidation. DECR2 participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. This protein catalyzes the NADP-dependent reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA. Recombinant human DECR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Peroxisomal 24-dienoyl-CoA reductase, Peroxisomal 2,4-dienoyl-CoA reductase, PDCR, SDR17C1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
33.2 kDa (315aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
De Nys K., et al. (2001) Biochim. Biophys. Acta. 1533:66-72; ; Daniels R.J., et al. (2001) Hum. Mol. Genet. 10:339-352
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_065715
Uniprot Accession Number
Q9NUI1
Species
Human
AA Sequence
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