Recombinant human PSAT1 protein

PSAT1, also known as phosphoserine aminotransferase, catalyses the conversion of 3-phosphohydroxypyruvate into 3-phosphoserine which is dephosphorylated subsequently by phosphoserine phosphatase to form L-serine. Recombinant human PSAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Phosphoserine aminotransferase, EPIP, PSA, PSAT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

42.9 kDa (394aa) confirmed by MALDI-TOF

Additionnal Information

PSAT1, Phosphoserine aminotransferase, EPIP, PSA, PSAT, ATGP1328-10 µg, ATGP1328-20 µg, ATGP1328-50 µg, ATGP1328-100 µg, ATGP1328-250 µg, ATGP1328-500 µg, ATGP1328-1 mg, ATGP1328-10, ATGP1328-20, ATGP1328-50, ATGP1328-100, ATGP1328-250, ATGP1328-500, ATGP1328-1

References & Citations

Choudhary C., et al. (2009) Science. 325:834-840; ; Hart C.E., et al. (2007) Am. J. Hum. Genet. 80:931-937

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_478059

Uniprot Accession Number

Q9Y617

Species

Human

AA Sequence

MDAPRQ VVNFGPGPAK LPHSVLLEIQ KELLDYKGVG ISVLEMSHRS SDFAKIINNT ENLVRELLAV PDNYKVIFLQ GGGCGQFSAV PLNLIGLKAG RCADYVVTGA WSAKAAEEAK KFGTINIVHP KLGSYTKIPD PSTWNLNPDA SYVYYCANET VHGVEFDFIP DVKGAVLVCD MSSNFLSKPV DVSKFGVIFA GAQKNVGSAG VTVVIVRDDL LGFALRECPS VLEYKVQAGN SSLYNTPPCF SIYVMGLVLE WIKNNGGAAA MEKLSSIKSQ TIYEIIDNSQ GFYVCPVEPQ NRSKMNIPFR IGNAKGDDAL EKRFLDKALE LNMLSLKGHR SVGGIRASLY NAVTIEDVQK LAAFMKKFLE MHQL