Recombinant human TRPT1 protein
TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
TRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.3 kDa (277aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hu Q.-D., et al. (2003) Cell. Mol. Life Sci. 60:1725-1732; ; Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001153862
Uniprot Accession Number
Q86TN4
Species
Human
AA Sequence
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