Recombinant human TRPT1 protein

TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

TRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.3 kDa (277aa) confirmed by MALDI-TOF

Additionnal Information

TRPT1, tRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1, ATGP1327-10 µg, ATGP1327-20 µg, ATGP1327-50 µg, ATGP1327-100 µg, ATGP1327-250 µg, ATGP1327-500 µg, ATGP1327-1 mg, ATGP1327-10, ATGP1327-20, ATGP1327-50, ATGP1327-100, ATGP1327-250, ATGP1327-500, ATGP1327-1

References & Citations

Hu Q.-D., et al. (2003) Cell. Mol. Life Sci. 60:1725-1732; ; Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001153862

Uniprot Accession Number

Q86TN4

Species

Human

AA Sequence

MNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ