Recombinant human Glutathione Peroxidase 2/GPX2 (U40C) protein

GPX2, also known as glutathione peroxidase 2, belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. GPX2 could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Glutathione peroxidase 2, GI-GPx, GPRP, GSHPx-2, GSHPX-GI, Selenoprotein GPX2, Gastrointestinal glutathione peroxidase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.1 kDa (210aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione Peroxidase2 (u40C), Glutathione peroxidase 2, GI-GPx, GPRP, GSHPx-2, GSHPX-GI, ATGP1325-10 µg, ATGP1325-20 µg, ATGP1325-50 µg, ATGP1325-100 µg, ATGP1325-250 µg, ATGP1325-500 µg, ATGP1325-1 mg, ATGP1325-10, ATGP1325-20, ATGP1325-50, ATGP1325-100, ATGP1325-250, ATGP1325-500, ATGP1325-1

References & Citations

Chu F.-F., et al. (1993) J. Biol. Chem. 268:2571-2576; ; Kelner M.J., et al. (2000) Gene. 248:109-116

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002074

Uniprot Accession Number

P18283

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAFIAKSFYD LSAISLDGEK VDFNTFRGRA VLIENVASLC GTTTRDFTQL NELQCRFPRR LVVLGFPCNQ FGHQENCQNE EILNSLKYVR PGGGYQPTFT LVQKCEVNGQ NEHPVFAYLK DKLPYPYDDP FSLMTDPKLI IWSPVRRSDV AWNFEKFLIG PEGEPFRRYS RTFPTINIEP DIKRLLKVAI