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Recombinant human IDH3G protein

IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD (+) as the electron acceptor, and is localised to the mitochondrial matrix. Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Isocitrate dehydrogenase [NAD] subunit gamma mitochondrial, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H-IDHG

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

41.1 kDa (375aa) confirmed by MALDI-TOF

Additionnal Information

IDH3G, Isocitrate dehydrogenase [NAD] subunit gamma mitochondrial, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H-IDHG, ATGP1324-10 µg, ATGP1324-20 µg, ATGP1324-50 µg, ATGP1324-100 µg, ATGP1324-250 µg, ATGP1324-500 µg, ATGP1324-1 mg, ATGP1324-10, ATGP1324-20, ATGP1324-50, ATGP1324-100, ATGP1324-250, ATGP1324-500, ATGP1324-1

References & Citations

Kim Y.-O., et al. (1999) J. Biol. Chem. 274:36866-36875; ; Brenner V., et al. (1997) Genomics. 44:8-14

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_004126

Uniprot Accession Number

P51553

Species

Human

AA Sequence

FSEQTIPPS AKYGGRHTVT MIPGDGIGPE LMLHVKSVFR HACVPVDFEE VHVSSNADEE DIRNAIMAIR RNRVALKGNI ETNHNLPPSH KSRNNILRTS LDLYANVIHC KSLPGVVTRH KDIDILIVRE NTEGEYSSLE HESVAGVVES LKIITKAKSL RIAEYAFKLA QESGRKKVTA VHKANIMKLG DGLFLQCCRE VAARYPQITF ENMIVDNTTM QLVSRPQQFD VMVMPNLYGN IVNNVCAGLV GGPGLVAGAN YGHVYAVFET ATRNTGKSIA NKNIANPTAT LLASCMMLDH LKLHSYATSI RKAVLASMDN ENMHTPDIGG QGTTSEAIQD VIRHIRVING RAVEA

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