Recombinant human IDH3G protein
IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD (+) as the electron acceptor, and is localised to the mitochondrial matrix. Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Isocitrate dehydrogenase [NAD] subunit gamma mitochondrial, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H-IDHG
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
41.1 kDa (375aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kim Y.-O., et al. (1999) J. Biol. Chem. 274:36866-36875; ; Brenner V., et al. (1997) Genomics. 44:8-14
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA
Scientific Category
Metabolism
NCBI Accession Number
NP_004126
Uniprot Accession Number
P51553
Species
Human
AA Sequence
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