Recombinant human SNRPC protein

SNRPC belongs to the u1 small nuclear ribonucleoprotein C family. This protein component of the u1 small nuclear ribonucleoprotein (snRNP) particle required for the formation of the spliceosome. This protein participates in the processing of nuclear precursor messenger RNA splicing. snRNP particles are attacked by autoantibodies frequently produced by patients with connective tissue diseases. Recombinant human SNRPC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

U1 small nuclear ribonucleoprotein C, u1C, Yhc1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.8 kDa (182aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

SNRPC, u1 small nuclear ribonucleoprotein C, u1C, Yhc1, ATGP1323-10 µg, ATGP1323-20 µg, ATGP1323-50 µg, ATGP1323-100 µg, ATGP1323-250 µg, ATGP1323-500 µg, ATGP1323-1 mg, ATGP1323-10, ATGP1323-20, ATGP1323-50, ATGP1323-100, ATGP1323-250, ATGP1323-500, ATGP1323-1

References & Citations

Sillekens P.T.G., et al. (1988) Nucleic Acids Res. 16:8307-8321; ; Muto Y., et al. (2004) J. Mol. Biol. 341:185-198

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003084.1

Uniprot Accession Number

P09234

Species

Human

AA Sequence

MPKFYCD YCDTYLTHDS PSVRKTHCSG RKHKENVKDY YQKWMEEQAQ SLIDKTTAAF QQGKIPPTPF SAPPPAGAMI PPPPSLPGPP RPGMMPAPHM GGPPMMPMMG PPPPGMMPVG PAPGMRPPMG GHMPMMPGPP MMRPPARPMM VPTRPGMTRP DR