Recombinant human PTK9/TWF1 protein
TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of 20 residues. The two ADF-H domains are 20% homologous to ADF/cofilin and to each other. Recombinant human TWF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Twinfilin-1, PTK9
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
31.5 kDa (277aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Beeler J.F., et al. (1994) Mol. Cell. Biol. 14:982-988; ; Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Cytoskeleton
NCBI Accession Number
AAH22344
Uniprot Accession Number
Q12792
Species
Human
AA Sequence
Frequently Asked Questions
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