Recombinant human PSMB2 protein
Proteasome subunit beta type-2, also known as PSMB2, is a member of the proteasome T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Recombinant human PSMB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome subunit beta type-2, HC7-I
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
24.9 kDa (221aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Morimoto Y., et al. (1995) J Biochem. 117:471-474.; ; Wnzel T., et al. (1995) Nat Struct Biol. 2:199-204.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002785
Uniprot Accession Number
P49721
Species
Human
AA Sequence
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