Recombinant human PSMA8 protein

Proteasome subunit alpha type-8, also known as PSMA8, belongs to the peptidase T1A family. PSMA8 is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit may be a testis-specific. Recombinant human PSMA8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome subunit alpha type-8, PSMA7L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30 kDa (270aa) confirmed by MALDI-TOF

Additionnal Information

PSMA8, Proteasome subunit alpha type-8, PSMA7L, ATGP1320-10 µg, ATGP1320-20 µg, ATGP1320-50 µg, ATGP1320-100 µg, ATGP1320-250 µg, ATGP1320-500 µg, ATGP1320-1 mg, ATGP1320-10, ATGP1320-20, ATGP1320-50, ATGP1320-100, ATGP1320-250, ATGP1320-500, ATGP1320-1

References & Citations

Schmidt M., et al. (1997) Mal Biol Rep. 24:103-112.; ; Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001020267

Uniprot Accession Number

Q8TAA3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK SVAKLQDERT VRKICALDDH VCMAFAGLTA DARVVINRAR VECQSHKLTV EDPVTVEYIT RFIATLKQKY TQSNGRRPFG ISALIVGFDD DGISRLYQTD PSGTYHAWKA NAIGRSAKTV REFLEKNYTE DAIASDSEAI KLAIKALLEV VQSGGKNIEL AIIRRNQPLK MFSAKEVELY VTEIEKEKEE AEKKKSKKSV