Recombinant human PSMA8 protein
Proteasome subunit alpha type-8, also known as PSMA8, belongs to the peptidase T1A family. PSMA8 is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit may be a testis-specific. Recombinant human PSMA8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome subunit alpha type-8, PSMA7L
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30 kDa (270aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Schmidt M., et al. (1997) Mal Biol Rep. 24:103-112.; ; Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001020267
Uniprot Accession Number
Q8TAA3
Species
Human
AA Sequence
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